Abstract

Patil Rahul Sahebrao, Prajakta Maskawade, Ragini Bundela*, Dr. Karunakar Shukla

ABSTRACT

The present work aims to study the potential effect of antioxidant rich methanolic leaf extract of Night Flowering Jasmine (MLNFJ) on colonic motility and histopathological observations in fructose (10%) induced hypertensive rats. The study was conducted on 60 Male Albino Wistar rats (110-150g). Rats were randomly divided into a group of six, each group containing 10 animals. Group I was considered as normal control which received chow pellets and normal drinking water ad libitum for 6 weeks. Group II received fructose (10%) solution instead of normal drinking water for 6 weeks. Group III, IV, V received fructose (10%) solution instead of drinking water ad libitum and MLNFJ at different doses (100, 200, 400 mg/kg p.o) for 6 weeks. Group VI received fructose (10%) solution instead of drinking water ad libitum and Enalapril at a dose of 10mg/kg p.o for 6 weeks. The cumulative concentration-response curve (CCRC) of Ang II was shifted towards the right by MLNFJ treatment using an isolated strip of rat ascending colon. MLNFJ treatment increased the contractile characteristics of the rat ascending colon in the CCRC of ACh as compared to the fructose-treated group. Histopathological changes observed in fructose (10%) group showed cloudy swelling in renal tubules, sclerotic glomerulus and renal hypertrophy. The liver of the fructose (10%) group showed the presence of macro vesicular steatosis, fat accumulation and congestion of blood sinusoids around the central vein. The aorta of the fructose group showed increased thickness of tunica media. Heart of fructose (10%) group shows vacuolation of cardiomyocytes. MLNFJ treatment reduced fructose-induced tissue damage due to the consequence of metabolic syndrome. The total phenolic content of MLNFJ was found to be 42.91μg gallic acid equiv./mg of extract. MLNFJ is rich in flavonoids and therefore has powerful antioxidant properties. The findings show that by battling oxidative stress caused by fructose (10%) and reducing Ang II activity, MLNFJ may be able to prevent the development of high blood pressure caused by fructose.