Abstract

Atin Srivastava, Pooja Sharma, Ragini Bundela* and Dr. Karunakar Shukla

ABSTRACT

Diabetes mellitus has become a major health concern worldwide and development and progression of diabetic related complications are accelerated by reactive oxygen species which are generated and accumulated due to hyperglycemia. herbal remedies are convenient for the management of diabetes due to their traditional acceptability and availability, low costs and lesser side effects. the present study was carried out to determine qualitative analysis of various phytochemical constituents, the in vitro antioxidant activity, and invitro and in vivo antidiabetic activity ethanol extract of leaves of feijoa sellowiana. qualitative analysis of phytochemical constituents was performed by the well-known tests protocol available in the literature and antioxidant activity was studied through DPPH assay, ABTS+ radical scavenging assay, nitric oxide scavenging method using ascorbic acid as standards. Antidiabetic activity was determined by in-vitro alpha-amylase inhibition assay and invitro antidiabetic activity was determined by diabetes was induced in rats by streptozotocin (100mg/kg; i.p.). The phytochemical screening revealed the presence of saponin, phenols, glycosides, flavonoids, terpenoids and alkaloids. The activities of ethanolic leaves extract against DPPH assay, ABTS+ radical scavenging assay, nitric oxide scavenging method were concentration dependent. In in-vitro antidiabetic test, highest α-amylase inhibition was found in ethanolic extract of feijoa sellowiana. Oral treatment of ethanolic extract of feijoa sellowiana using rat oral needle at 200 and 400mg/kg doses significantly (???? < 0.001) decreased blood glucose levels. In conclusion, the above actions might be responsible for the antidiabetic activity of extract due to presence of gallic acid and other biomarkers.