Abstract

Tanvi Gupta*, K. K. Badoniya and Surendra Jain

ABSTRACT

The use of herbal plant extracts in wound healing is known through decades, but it is necessary to provide scientific data through reverse pharmacology. The present research aims to formulate and evaluate an herbal gel containing Calendula officinalis and Ixora chinensis flowers methanolic extract. The extracts were subjected to various physicochemical evaluations. The present study was to determine qualitative phytochemical analysis, in vitro antioxidant, anti-bacterial and wound healing activities. Qualitative phytochemical screening of Calendula officinalis is showed the presence of active metabolites such as alkaloids, flavonoids, proteins, tannin, saponin, protein, glycosides and phenols is presented and Ixora chinensis showed the presence of active metabolites such as flavonoids, steroids, carbohydrates, phenols and alkaloids. DPPH radical scavenging activity of Calendula officinalis extract exhibited percent inhibition 60.32% and its IC50 value was found to be 49.38μg/ml and Ixora chinensis extract exhibited percent inhibition 60.15% and its IC50 value was found to be 49.56μg/ml. Ascorbic was used as a reference compound which exhibited percent inhibition 85.16% and showed IC50 value of 22.67μg/ml. The pH of all prepared formulation ranged from 6.3-6.7. The measurement of viscosity of the prepared gel were found to be formulation 1-5455, formulation 2-5509 and formulation 3-5691 cps. Spreadability of different gel formulation was studied. The formulations produced good spreadability. Skin irritation test indicate that prepared gels were not produce irritation, redness, or edema on application and free from dermatological reaction. Antibacterial activity was performed against E. coli by well diffusion assay. Formulation 3 and standard gel showed best zones of inhibition. Further we were performed the wound healing studies like contraction of wound model for 21 days. The wound contraction studies revealed that the wound contraction increases on increasing the concentration of herbal extract. The study also reveals that the better activity of polyherbal formulation may be due to the synergistic action of the plant’s constituents present in the formulation. Thus, the prepared topical gels possess a versatile approach in healing the wound contraction.