Abstract

Aslam Farheen*, Najeeb Maham, Mohsin Sahar, Abdul Manan Laiba and Iftikhar Saima

ABSTRACT

This study investigated the isolation and characterization of collagenase from chicken bone. Collagenases have widespread applications in food industry and clinical therapies. This enzyme hydrolyses collagen by breaking down peptide bonds at different sites. In this research, collagenase producing bacteria were isolated by repeated streaking method. Morphological examination confirmed the classification of the bacterial isolates as Bacillus sp. The parameters such as time of incubation, pH, substrate concentration, metal ions, carbon and nitrogen sources were also optimized for maximum production of the enzyme. The optimal time of incubation for enzyme activity was 48 hours. The maximal enzyme activity was obtained at pH 8.0 and optimum substrate concentration was 2% gelatin. The results revealed that MgSO4, CaCl2, glucose, and beef extract are best sources of metal ions, carbon, and nitrogen for the maximum production of enzyme. Our research work has investigated those bacteria that are producing collagenase enzyme with greater activity.