Abstract

Aslam Farheen*, Ahmed Zumar and Iftikhar Saima

ABSTRACT

Beta glucanase is most important enzyme that offer significant benefits, includingimproved brewing efficiency and product clar ity in the food and beverage industry,enhanced nutrient absorption and gut health in animal feed. The highestextracellular activity was observed at pH 8 (0.95±0.11 IU/ml), while the highestintracellular activity was observed at pH 9 (0.8±0.73 IU/ml). Bo th activities peakedat 24 hours (0.51±0.20 IU/ml) and 48 hours (1.16±0.13 IU/ml) of incubation forextracellular and intracellular respectively. Glucose produced both the highestextracellular (1.51±0.92 IU/ml) and intracellular activity (1.13±0.98 IU/ml) ml).MnSO4 enhanced both extracellular (1.16±0.14 IU/ml) and intracellular (1.08±0.50IU/ml) activity. The best nitrogen sources were Beef extract (0.98±0.22 IU/ml), andpeptone (0.45±0.14 IU/ml) shows highest activity for intracellular and extracellularresp ectively. This research work has investigated bacteria which is producing betaglucanase enzymes with greater activity. For determining 3D structure of enzymePhyre2 and Swiss model was used that predicted the structure of beta glucanase.