Abstract

Aslam Farheen*, Umar Fatima and Iftikhar Saima

ABSTRACT

Collagenases play a crucial role in treating multiple diseases such as Dupuytren's disease and Peyronie's disease and medical problems. Collagenases are responsible for the degradation of native collagen to small peptide fragments. It has wide applications in the food industry as meat tenderization, cosmetics, wound healing, diabetic ulcers, arterial ulcers, etc. Collagenases are mostly obtained from Clostridium histolyticum. In this research, the source used was soil from which the enzyme was extracted, and the culture was purified by the streaking method, further TCA solution was used to produce zones around colonies which showed the hydrolysis of gelatin, a substrate for collagenase. Biochemical testing confirmed the presence of Bacillus sp. and it is a good source of collagenase. The bacteria was confirmed by bioinformatics analysis by predicting its structure. The enzyme activity exhibited the highest incubation period of 24 hours with other parameters such as ammonium hydrogen citrate as a nitrogen source, glucose as a carbon source, sodium chloride as a metal ion, and a maximum substrate concentration of 2 %. This research shows an optimization of characterization for maximum production of collagenase.