ISOLATION AND CHARACTERIZATION OF Β-GALACTOSIDASE PRODUCING BACILLUS SUBSTILIS ISOLATED FROM RAW MILK
Rashika Yousaf, Farheen Aslam*, Hafsa amat ul Rasool and Saima Iftikhar
ABSTRACT
This research is based on production of beta-galactosidase from bacillus strains of bacteria. Beta- galactosidase is important enzyme that can be produced commercially by using bacterial strains. This enzyme hydrolyze lactose into products glucose and galactose. Beta-galactosidase has various industrial applications like treatment of lactose mal absorption and lactose hydrolyzed milk. In present study, beta-galactosidase was isolated from raw milk source by using repeated streaking method. By using 16S ribosomal sequencing, Bacillus substilis was detected that produce beta-galactosidase enzyme in nature. This bacterium showed best activity at 37°C optimized temperature, optimized time of incubation was found to be 48 hours, optimum pH with best activity was around 6 and 7 and optimum substrate concentration was 1.5% lactose. Beta-galactosidase enzyme was extracted by using different saturation levels of ammonium sulphate and then dialysis was performed. For determining the size of protein, SDS PAGE was performed. Present research gives valuable insight regarding β-galactosidase production by Bacillus substilis present in raw milk.
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