Abstract

Shyni Bernard*, Neenu Kuriakose, Amrutha P. Anil, Sumayya C. T., Ayana B., Shilpa M. K. and Bindu R.

ABSTRACT

Background: Hydrotropic solubilization is an organic solvent-free approach for poorly water soluble drugs. Abiraterone acetate is a poorly water soluble anti-androgenic drug used to treat prostate cancer. Literature survey shows that various method such as HPLC, UPLC, LC-MS/MS and UV spectrophotometric method using methanol as solvent have been reported for the estimation of Abiraterone acetate. In this work, the selected hydrotropic agent sodium lauryl sulphate was employed to solubilize the drug, replacing harmful organic solvents. Method: The UV spectrophotometric analysis was carried out at 255 nm, the maximum absorption wavelength of Abiraterone acetate. Beer?s law was found to be obeyed in the concentration range of 10-45μg/mL. The correlation co-efficient for Abiraterone acetate was found to be 0.99973.The results of analysis have been validated as per ICH guidelines. The molar absorptivity was calculated as 2.2244 X 104 l/mol.cm. Validation parameters such as accuracy, precision, and recovery were assessed, shows the recovery rates between 99.94 % to 99.96% and relative standard deviations below 2%. The proposed method was found to be accurate, eco-friendly and reproducible. The developed method can be applied to the routine quality control of Abiraterone acetate in tablet formulations.