A REVIEW ON IMMUNOBLOTTING TECHNIQUES
Sakshi C. Sapte, *Swarup G. Salunke, Srushti V. Saindar, Aman S. Shaikh, Saad S. Shaikh and Manjusha P. Mhaske
ABSTRACT
Blotting techniques such as Western, Southern, and Northern blotting are essential molecular biology tools used for detecting specific proteins, DNA, and RNA, respectively. Among them, immunoblotting (Western blotting) is widely utilized for protein analysis due to its high specificity, combining electrophoresis, membrane transfer, and antibody-based detection. This review outlines the principles, procedures, and applications of each blotting method, highlighting their roles in diagnostics, gene expression studies, and biomarker discovery. Additionally, the limitations of immunoblotting are discussed alongside advancements aimed at improving sensitivity, throughput, and reproducibility. These techniques continue to evolve, maintaining their significance in both research and clinical settings.
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