EXTRACTION OF NARINGIN BY USING TWO DIFFERENT METHODS AND ANALYZING THROUGH UV SPECTROMETRY
*Lakpa Rinzing Sherpa
ABSTRACT
Naringin, a flavanone glycoside abundant in citrus peels, possesses diverse pharmacological properties. This study compared solvent efficiency for extracting naringin from Citrus reticulata peel using maceration with methanol, ethanol, dimethylformamide (DMF), and dimethyl sulfoxide (DMSO), and Soxhlet extraction with ethanol. Two grams of powdered peel were macerated for 72 hours in each solvent, while Soxhlet extraction was carried out on 30 g peel for 4 hours. Extracts were analyzed spectrophotometrically at 276 nm against a naringin standard. DMF showed the highest absorbance (0.600), yielding approximately 2.47 mg per 2 g peel (1.24 mg/g), followed by DMSO (0.491 mg per 2 g). Methanol and ethanol gave lower yields. Soxhlet extraction produced a higher crude extract (0.9 g from 30 g; 3% w/w), but naringin isolation was not quantified. Overall, DMF proved to be the most efficient solvent for naringin extraction under these conditions.
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