Abstract

Dr. Ganesh Akula*, Bandoj Nikhitha, Manumula Jusya Vani, Jonnada Swetha, Saniya Begum, Joganolla Sri Vidya

ABSTRACT

A simple, rapid, and reliable UV–visible spectrophotometric method was developed and validated for the quantitative determination of lornoxicam using 40% (v/v) methanol in phosphate buffer pH 7.4 as the solvent system with detection at 378 nm. The developed method exhibited excellent linearity over the concentration range of 2.5–25 µg/mL with a regression equation of y = 0.0404x + 0.0042 and a correlation coefficient (r) of 0.9997 ± 0.006, confirming compliance with Beer–Lambert’s law. Precision studies demonstrated high repeatability with intra-day relative standard deviation (RSD) values ranging from 0.222% to 0.480% and inter-day RSD values between 0.904% and 2.41%. Accuracy of the method was confirmed through recovery studies at three concentration levels (15, 20, and 25 µg), yielding mean recoveries in the range of 99.35–99.88% with RSD values below 0.63%. Stability studies indicated that lornoxicam solutions remained stable for up to 72 hours under both refrigerated conditions (8 ± 1°C) and laboratory temperature (25 ± 1°C), with RSD values of 0.987% and 0.865%, respectively. The method was successfully applied to the analysis of marketed tablet formulations, showing no interference from pharmaceutical excipients and yielding drug content of 99.46% with an RSD of 0.94%. The validated method was also effectively employed for the quantification of lornoxicam in solid lipid nanoparticle (SLN) formulations. Owing to its simplicity, accuracy, precision, reproducibility, and cost-effectiveness, the developed UV spectrophotometric method is suitable for routine pharmaceutical analysis and quality control applications.