Abstract

Ali Rezvani Dehaghani*, Sourena Rezvani Dehaghani, Dr. Najmeh Ghanbari

ABSTRACT

Aim: in the present study, anti-metastatic and toxicity effects of cerium oxide nanoparticles on the MCF-7 and T47D breast cancer cell lines were determined. Material and Method: T47D and MCF-7 breast cancer cell lines and HEK293 normal cell lines were treated with 6.5 mg, 65 and 650 ?g and 65 and 650 ng of cerium oxide nanoparticles. MTT analysis was used for the evaluation of nanoparticles toxicity effect. The expression level of MN23 and KAI-I genes were measured by real-time PCR method. Results: In the 650 ?g and 6.5 mg concentration of CNP, nearly 60% of MCF-7 and T47D were dead, respectively. Also, in the normal cells treated with 6.5 mg of CNP, survival reduced by 25%. According to the gene analysis results, the expression of NM23 and KAI-I anti-tumor genes was not increased in the presence of CNP. Conclusion: Based on the present study results, the toxic effect of cerium oxide nanoparticles on breast cancer cells was confirmed. However, the effect was dose and cell line depended. On the other hand, evaluation of anti-metastatic genes expression showed that the cerium oxide nanoparticles could not elevate gene expression and therefore ineffective in controlling cell invasion and probably induced anti-cancer effects via the apoptosis pathway.