Abstract

Ali Osman Albayrak*, Funda Karabağ Çoban, Mehmet Emrah Şelli and Hande Aytuğ

ABSTRACT

Breast cancer is the most common type of cancer among women and ranks second among the causes of female death in the world. In order to find a solution to breast cancer, different studies are being conducted for the treatment and the effects of different drugs and substances on this disease are intensively investigated. Boric acid has been shown to control the proliferation of certain types of cancer cells. In the study, different concentrations of boric acid were applied on MCF-7 cell line to determine IC50 values of boric acid and its antiproliferative effect was determined using Cell Counting Kit-8 (CCK8) ELISA kit at 24, 48 and 72 hours. According to the results, other analyzes were performed over the 48 hour incubation period. After the IC50 values of boric acid at 48 hours were determined, Poly ADP-ribose polymerase (PARP) kit was used to determine apoptotic effects for boric acid. Cell lysates were obtained after the application of defined IC50 doses to the cells for determination of biochemical parameters. The vascular endothelial growth factor (VEGF) kit was used to determine the angiogenic effects with the obtained samples. As a result, in this study, it was observed that concentration and time-dependent boric acid decreased proliferation in MCF-7 breast cancer cell line. At the same time, according to the PARP results, it was found that the concentrations of boric acid significant differences compared to the control group. There was no significant difference between boric acid concentrations and Cis Platin groups. It is thought that both groups showed an increase compared to the control group, and boric acid leads the cell to necrosis like Cis Platinum. When VEGF results were examined to explain the relationship between boric acid and angiogenesis, significant differences were observed with both control group and Cis Platinum. Boric acid groups have been shown to be more effective than Cis Platin in the MCF-7 breast cancer cell line and to be an antiangiogenic agent. In this respect, further studies are needed to investigate how the effects of molecular anticancerogenic mechanisms of MCF-7 cells and boric acid on cell polarization are realized.