Abstract

Madhan Mohan T., P. Arul Prakash and A. Jayachitra*

ABSTRACT

Lupeol, a triterpene found in plant source like fruits and vegetables which selectively induced apoptosis of cancer cells. It also regulates several apoptosis signaling pathways in cancer cells. It has downregulated the proteolysis activity of secreted MMP-2 protein suggesting the efficacy of Lupeol against the spread of Cancer cells. Its role in induction of pro-apoptotic protein indicates that Lupeol rectifies the errors in apoptotic machinery of cancer cells. Lupeol treatment showed cell growth inhibition, anti-inflammatory effects, and tumor regression using FACS for cell cycle analysis, apoptosis, ROS. Furthermore, Lupeol showed antiproliferative action towards U87MG cells at the Inhibitory concentration of 33.76?M. On cell cycle analysis, Inhibition of cells at various stages by lupeol was observed between control and treated. The vast difference in peak count revealed with treated sample count at 300(peak 1) & 240(peak 2) whereas control count at 440(peak 1)& 300(peak 2). The apoptosis inducing activity of lupeol on U-87MG cells shows mean difference treated at 64.982 compared to control at 80.823. Lupeol also regulated the reactive oxygen species in the cells in which excessive distribution occurred at the range of 50-150. These studies conclude that lupeol has the ability to inhibit and induce the Apoptosis, ROS. Thus, confirming anti tumor properties of lupeol.