Abstract

Hussam Aldeen Mustafa Abdelrazig Bilal*, Elniema A. Mustafa, Qusai Hussain Abdel Samad and Adil M. A. Salman

ABSTRACT

This descriptive and experimental study was conducted between January and Aril, 2020 in Lean slaughterhouse, Alkadro, Khartoum North, Sudan using a regular swab sampling on a weekly basis for a period of 3 weeks with the objective to evaluate cleaning and disinfection procedures against some microbial isolates in the slaughterhouse. Routine cleaning and disinfection procedures which included walls, floors and equipment using hydrogen peroxide solution in 0.01 concentration were investigated. Both environmental swab samples and standardized and structured checklist were applied to assess the status of some of the Prerequisites Programs (PRPs). The study revealed that the location and structure of the slaughterhouse and staff training were found satisfactory while water supply had failed adequacy rule. According to adequacy rule used in this study all the parameters of sanitation program operational procedures scored satisfactory (100%). While the average of averages of coliform count in the floor, wall, equipment and water was found 2733.3±540.6 cfu during the three weeks period with significant statistical differences in the count between the three weeks with p ?0.05. The average of Total Bacterial Count for the sample sites during the three weeks period was 2250±680.6 cfu, with statistical differences in the count between the three weeks with p ?0.05.The average of E. coli count was 341.6±85.6 cfu for the sample sites during the three weeks period with statistical differences in the count between the three weeks with p ?0.05. The multiple comparison test of least significant differences showed no significant differences in the Salmonella count between floor, wall, and equipment with p ?0.05. The mean TBC differences was found to be significant between wall and floor and wall and equipment with p ?0.05; also the mean differences of E. Coli count was found to be significant between wall and equipment from one side and floor and equipment from the other side with p ?0.05, and the mean Coliform count differences was found to be significant between the wall and equipment with p ?0.05. The study concluded that the overall evaluation of the PRPs and sanitation program operational procedures were satisfactory.