Abstract

Dr. Mradu Gupta*, Dr A. K. Mondal, Dr. K. A. Ahmed

ABSTRACT

An Ayurvedic formulation prepared from dried root powder of Sida cordifolia Linn. is standardized through pharmacognostical and phytochemical pre-clinical studies for treatment of male sexual disorders. While total ash value was 8.0%, acid insoluble ash was 0.5% and water soluble ash was 5.41%. Among elements, Nitrogen was found present. Flavonoids and carbohydrates were found present in both alcoholic and aqueous extracts. The results also showed high concentration of flavonoidic compounds (15.03 ?g Quercetin equivalent / mg of extract) in the alcoholic extract and high phenolic content (23.26 ?g Gallic acid equivalent / mg of extract) in the aqueous extract. HPTLC analysis at 280 and 360 nm indicated the presence of Ellagic acid and Gallic acid in both these extracts. Similarly, HPLC analysis at 276 nm showed elution of 4 compounds whose analysis confirmed the presence of Tannic acid, Benzoic acid and Quercetin in aqueous extract. UV-Visible spectroscopy scanning showed peaks at 896, 862.5 and 195.5 nm in aqueous and at 896, 862.5, 419.5 and 209.5 nm in alcoholic extract. FTIR analysis indicated presence of N-CH3 out-phase bending, N-CH3 in-phase stretching, Aryl -CH3 in-phase stretching and C-O stretching functional groups in aqueous and -NH2 Stretching, -CH2 in-phase stretching, (N)-CH3 in-phase stretching, -CH3 in-phase bending, Aryl-N stretching and N-O stretching functional groups in alcoholic extract suggesting the presence of amides, aldehydes, alkaloids and phenolic groups of compounds in the extracts. The presence of phenolic and flavonidic compounds in research formulation extracts may be responsible for its antioxidant, analgesic, antipyretic, anti-inflammatory and antimicrobial properties.