Abstract

C. Pramod*, Dr. Ratheesh M., Svenia P. Jose and Feena Paul

ABSTRACT

Methods: The bark was shade dried and coarsely powdered and then subjected to hot continuous percolation by Soxhlet apparatus using ethanol as solvent. Preliminary phytochemical analysis was carried out on extract. The antioxidant, anti-inflammatory and antiarthritic potential was evaluated by in vitro and in vivo methods. In vitro methods include Nitric oxide scavenging assay, DPPH assay, Protein denaturation and proteinase inhibition methods. In vivo antiinflammatory activity was established by using carrageenan induced paw edema and cotton wool granuloma methods. In vivo antiarthritic potential was evaluated by collagen induced arthritis model. After 20 days of collagen administration, EEBL 200mg/Kg was administered to experimental Sprague dawley rats for next 20 days. Changes in body weight, paw volume, arthritic score were assessed weekly. In addition, on 41st day, radiological analysis, histopathological evaluation, TNF-? expression by RT PCR analysis were performed. Results and Discussion: In vitro studies showed that EEBL had got good nitric oxide and DPPH scavenging activity and also possess significant antiinflammatory activity. In in vivo studies, extract treatment in experimental rats at the dose of 200mg/Kg showed a decrease in the paw volume and granuloma weight indicating the possibility of EEBL be a promising antiinflammatory agent. Studies on EEBL treated collagen induced arthritic animals, showed an increase in body weight, significant reduction in paw volume, arthritic score and TNF-? expression. Radiological and histopathological analysis also strengthens the evidence of resolution of arthritis. Conclusion: The study confirmed the antiinflammatory and antiarthritic potential of Buchanania lanzan Spreng. bark belongs to the family Anacardaceae.