Abstract

M. K. Ranganath, Kalyani Arikatla*, Prasanta Deka

ABSTRACT

The main objective of this study is to develop a reverse phase HPLC method for the simultaneous determination of candesartan and amlodipine bulk and pharmaceutical dosage form with a simple, rapid, specific, validated and sensitive method. An isocratic separation is achieved using C18(150 x 4.6mm, 5?) with mobile phase comprises of water and methanol in the ratio of 10:90 v/v. candesartan shows a retention time 3.5min and amlodipine shows 1.17min at 1ml/min flow rate and the wavelength was detected at 355nm. Robustness, specificity, precision, accuracy, linearity, LOD and LOQ was validated using this method. The LOD and LOQ are 0.48 and 1.5 for amlodipine and 0.75 and 2.3 for candesartan respectively. The calibration curve in the concentration range of 4-24 mcg/ml for both AMLO and CANDE are linear with the coefficient of correlation 0.997 and 0.998 respectively. The % of recovery of candesartan is 99.5% and amlodipine is 100.3% and the % od RSD is <2%. This method is successfully applied for quantitative determination of candesartan and amlodipine bulk as well as the formulation.