Abstract

Mohammed Hassan Flaih*, Fadhil Abbas Al-Abady and Khwam Reissan Hussein

ABSTRACT

The study includes three local locations, Al-Hussein Teaching, Suq Al-Shyokh General and Al-Shatrah General Hospitals in province for the period from December 2018 to September 2019. The samples were collected from 80 patients suffering from cutaneous leishmaniasis (CL). Nested-PCR was used to amplify kinetoplast minicircle fragments DNA (kDNA). Also, 3ml of the venous blood was collected before they take a drug, then putted in EDTA tube for order complete blood count (CBC) test. Electrophoresis results for 80 samples of kinetoplast minicircle fragments DNA gene were amplified by Nested PCR, discovered 65 positives for cutaneous leishmaniasis and Furthermore, the results were recorded 46 (57.5%) positive samples of L. tropica at 750bp. In present study, there are not significant differences of erythrocytes count and hemoglobin concentration by compared between patients and control, whereas platelet blood count showed a significant increase. On the other hand, the statistical analysis findings were showed significant differences by compared between leukocytes count and WBC differential (neutrophils, lymphocytes, eosinophils and basophils) of patients group and control specimen, except of monocytes which showed an insignificant difference at compared between patients group and control.