Abstract

Kandanath B. M.*, Thomas B. T. and AlKhalaf M.

ABSTRACT

Introduction: Acetonitrile(ACN) is completely miscible with water and its high dielectric constant and dipole moment makes it suitable solvent for many inorganic and organic compounds. The solvent has a medium polarity and a weak hydrogen bond acceptor. The objective of this study is to know how different dilutions of ACN(Acetonitrile) will affect the concentration and purity of primers eluted through the column. Method: Trityl off synthesized B-actin primers in triplicates were eluted through the size exclusion columns which is prewashed with the wash buffer. Different dilutions of ACN were prepared with with a polar solvent to look at the concentration and purity of eluted primers. Results: 50% ACN eluted primers were giving an average concentration of 591ng/ul and purity of 1.8(260/280) than that of 100% and 20% CAN eluted primer with an average concentration of 297ng/ul and 189ng/ul and purity of 1.72 and 1.5 respectively. Conclusion: Trityl off primers were eluted in higher concentration and purity in 50% ACN as the elution buffer through the gel filtration columns.