Abstract

Mithin U. C., Rinku Buragohain, Tapas Kumar Sar* and Tapan Kumar Mandal

ABSTRACT

Background: The Extended spectrum ? lactamases (ESBLs) producing organisms are highly prevalent in poultry and poultry products. These ESBLs producing bacteria can hydrolyze ? lactam antibiotics (like ceftriaxone, cefotaxime) which may result in treatment failure and development of antimicrobial resistant. Objective: The study was conducted to evaluate the disposition kinetics of ceftriaxone (3rd generation cephalosporin) and tazobactam (beta-lactamase inhibitor) in broiler, Rhode Island Red and Haringhata black poultry following single dose intramuscular administration and to evaluate the possible use of ceftriaxone- tazobactam combination (8:1) for treating resistant bacterial infections in poultry. Method: A single dose of ceftriaxone @ 25 mg kg-1 and tazobactam @ 3.125 mg kg-1 was administered intramuscularly to eighteen poultry (six poultry each in broiler, Rhode Island Red and Haringhata black breed) and blood samples were collected at predetermined time interval for HPLC analysis. Result: Both ceftriaxone and tazobactam persisted up to 6 h in plasma of all three breeds of poultry without showing significant inter-breed variations. The plasma concentration of ceftriaxone maintained above the minimum inhibitory concentration of 0.12 ?g mL-1 required against TEM-1 containing Escherichia coli. The pharmacokinetic study revealed that both ceftriaxone and tazobactam was absorbed quickly from intramuscular absorption site with significant volume of distribution in all three breeds of poultry. The plasma concentration also maintained a 8:1 ratio for ceftriaxone and tazobactam. Conclusion: Therefore ceftriaxone @ 25 mg kg-1 and tazobactam @ 3.125 mg kg-1 may effectively be used as 8:1 combination to treat ESBL producing bacterial infection in poultry.