Abstract

Senthamizhselvi S., Angelin Buela Caroline M., *B. K. Nayak and Anima Nanda

ABSTRACT

Actinomycetes, one of the exciting novel group of filamentous bacteria are well documented for their metabolic adaptability. The bioactive feasible of these actinobacteria enables their survival even in anguish and unfavourable environments. Different unexplored environments often demand to researchers in the hope of ensuing novel actinomycetes with nonstop mission which has really led to find remarkably industrious microbial strains. In our current study, actinomycetes were isolated from different indoor environments i.e., Botany staff room, Toilet, Library, Laboratory and outdoor environments like Basketball ground of our KMGIPSR college campus by Gravity Petriplate sedimentation method with the use of five media. A total of 16 actinomycetes isolates were recorded from both the environments. Among the Petri plate exposer timing, 60 second exposer was found to be good to isolate a countable number of actinomycetes in comparison to 30 second and 10 second. Glycerol Asparagine Agar (GAA) was recognised as one of the compatible media to isolate actinomycetes from air compared to Starch Casein Agar (SCA) and Actinomycete Isolation Agar (AIA). In concentration, isolated airborne actinomycetes were found to be same together in all the indoor and outdoor environments of the college campus. Based on the antagonistic nature on agar plates, 5 actinomycetes were tested for their antimicrobial potential, only two actinomycetes were found active against E. coli, Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumonia and Candida albicans.