Abstract

Anzaku S.* and Ishaleku D.

ABSTRACT

Antibiotic susceptibility and molecular detection of fluoroquinolones resistance genes from Salmonella typhimurium isolated from Poultry farms in Keffi, Nigeria. A total of one hundred and sixty (160) samples of feacal droppings were collected from different poultry farms and Salmonella typhimurium were isolated and identified using standard microbiological methods. Fluoroquinolone resistant genes (parE, gyrB and gyrA) were carried out using polymerase chain reaction (PCR) method. The occurrence of Salmonella typhimurium was 24.4%. The occurrence of Salmonella typhimurium in respect to different poultry farm was higher in farm C (32.5%) and lower in farm D with (15%). In this study Antibiotics Resistance shows that Salmonella isolates were resistant to Ciprofloxacin, Cefotaxime and Ofloxacin. Antibiotics resistant phenotype against Salmonella isolate were distributed into different antibiotic resistance pattern and the most common pattern were STX-CTX-NOR-AMC-CXM-CIP-C with 12.8% Resistance and CTX-NOR-AMC-CXM-TET-CIP-C with 1.03% Resistance. The multiple Antibiotic Resistance (MAR) Index of Salmonella species isolated was common at ?0.7 with 38.5%. fluoroquinolone resistant genes gyrA, gyrB, and parE were detection from isolates that were highly resistant to quinolone drugs. Poultry farmers and handlers should ensure proper hygiene to avoid contamination in poultry farms and poultry products.