UV-VISIBLE AND FTIR SPECTROSCOPIC ANALYSIS OF THE CRUDE ETHANOLIC EXTRACT OF PEURARIA PHASEOLOIDE LEAF (ROXB) BENTH. (FABACEAE)
Onyemailu V. O., *Bruce S.O and Iloh E. S.
ABSTRACT
The present study was carried out to characterize the bioactive constituents present in the Ethanolic extract of Peuraria phaseoloides leaves using UV-Vis and FTIR spectroscopic techniques. UV-VIS method was performed using a Perkin-Elmer Lambda 19 spectrometer while the FTIR method was performed using Buck scientific M530 system which was used to detect the characteristic peak values and their functional groups. UV-Visible spectrum revealed varying peaks in the range of 220-900nm recording the absorptions at its respective wavelengths. It showed a ?max peak at 500nm, with peaks at 420, 440 and 460nm indicating the presence of flavonoids. The FTIR analysis revealed the highest peak at 3291.098cm-1, which signifies the presence of R2NH 20 amine functional group. Other functional groups such as Halogen, ether, ethane, carboxylic acid, carbonyl, nitrile and alcohol were equally present. The presence of various functional groups and phytocompounds in Peuraria phaseoloides confirm that it act as a most important source of drugs against various ailments.
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