Abstract

Praveen Kumar Dasari*, Gannamaneni Jagruthi Sai, Bathula Naga Vara Laxmi Kumari, Medidhi Keerthana, Tanuku Satyanarayana

ABSTRACT

The present research work deals with determination of phytochemical constituents and anthelmintic activity of Thuja occidentalis leaves of Cupressaceae family were identified in order to relate their presence with bioactivities of the plants. The research find highlights that methanolic extracts of Thuja occidentalis had the highest number of phytochemicals compared to other solvent extracts. Hence, methanolic extracts of Thuja occidentalis holds the greatest potential to treat various human diseases and has profound medical applicability. In-vitro anthelmintic activity was evaluated by taking adult Indian earthworms, Pheretima posthuma having anatomical and physiological resemblance with intestinal roundworms. The earthworms were washed in normal saline solution before they were placed in to 10ml of respective formulation. To observe anthelmintic activity, all the investigations were carried out by methanolic extract with different concentrations of 10, 20, 50mg/ml, significant activity like time of paralysis and time of death were noted. At highest concentrations of 50mg/ml, a significant anthelmintic activity was observed and compared with piperazine citrate (10, 20, 50mg/ml as standard reference and distilled water as control. Herbal drugs and synthetic drugs were equally effective in helminthic infestations but methanolic extract of Indian medicinal plants exhibits potentiality and have maximum anthelmintic activity.