Abstract

*Dr. Dhanapal Venkatachalam

ABSTRACT

Objective: The objective of the study was to evaluate in-vitro Anti-inflammatory activity of Ethanol and aqueous extracts of whole plant of Coldenia procumbens. It is an annual herb belonging to Boraginaceae family. In the traditional system of medicine, the plant was used as anti-inflammatory, anti-microbial, analgesic, anti-diabetic, CNS depressant, hepato protective activity, anti-oxidant activity. Methodology: Ethanol and aqueous extracts of whole plant of Coldeniaprocumbens were used for Anti-inflammatory activity. The ethanol and aqueous extracts of the whole plant of Coldeniaprocumbens were studied for anti-inflammatory activity by protein denaturation, Human Red blood cell membrane stabilization and albumin denaturation method with reference to aspirin. Results: The anti-inflammatory activity of ethanol and aqueous extract of Coldeniaprocumbens with reference to aspirin was shown in the parameters. The percentage of inhibition of protein denaturation and percentage of membrane stabilization for ethanol extracts and aspirin was done at 100?g/ml. The ethanol extract of C.procumbens shows anti-inflammatory activity at concentration 1000 ?g/ml shows 94% protection (inhibit protein denaturation) and 98% protection (Human red blood cell membrane stabilization). The aqueous extract of C.procumbens shows anti-inflammatory activity at concentration of 1000 ?g/ml shows 89% (inhibition of protein denaturation) and 95% protection Human red blood cell membrane stabilization). Conclusions: The result concludes that the ethanol and aqueous extracts of Coldeniaprocumbens possess anti-inflammatory activity properties and further the plant can be explored for isolation of its active constituents.