PRIMARY SCREENING OF ANTIMICROBIAL ACTIVITY USING ENDOPHYTIC ACTINOMYCETES FROM AVICENNIA MARINA (FORSK). VIERH
Thiruvarasan K., Ganesan T., Nithiya G., Nayak B. K.* and Anima Nanda
ABSTRACT
During the present study, actinomycetes were isolated from endodermal regions of the leaves of mangrove plant, Avicennia marina by serial dilution and agar plate method. All the twenty one isolated actinomycetes were curtained for their antagonistic properties against pathogenic bacterial strains procured from IMTECH, Chandigarh, India. Initially the actinomycetes were primarily screened by cross streak method and only two potential actinomycetes were tested subjected to secondary screening which was done by agar well diffusion method. Seven isolates were found to prevent the pathogenic bacteria but Avp-5 isolate was established as active against S. aureus, S. epidermis, K. pneumoniae, P. vulgaris, B. subtilis, E. coli, S. pyogenes and MRSA but Avp-2 showed its potential against all pathogens other than S. epidermis, K. pneumoniae. Few of the endophytic actinomycetes (around 30%) showed good antagonistic effect against most of the tested pathogens and 70% of the total actinomycetes were found non-effective against the test pathogens.
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