Abstract

*Sandhya E., Sankari M. and 3Dr. Meena A

ABSTRACT

The study designed for identification of the anti-angiogenesis and anti-cancer activity of ethanolic extract of Spathodea campanulata root bark (EESC). The phytochemical chemical studies showed that the presence of phenolic and flavonoid constituents exerts antioxidant and free radical scavenging activity. The Pharmacognostical evaluation of EESC by fluorescence analysis with ethanol showed dark red in day light, black in short UV (254nm) and brown in long UV (366nm). The pharmacological assay includes in-vitro MTT assay and DNA fragmentation assay using MCF7 breast cell lines. The result revealed that the cell viability of MCF7 breast cell lines was 51.19% and IC50 value calculated for EESC was 62.5?g/ml which indicates that the presence of cytotoxic activity by MTT assay and DNA ladder formation indicated the cytotoxic effect of EESC caused by inhibition in the growth of breast cancer through apoptosis using DNA fragmentation method concludes the use of anti-cancer agent. In-ovo Chick Chorioallantoic membrane (CAM) assay includes 60 Fertile white leghorn eggs and on the sixth day of incubation 48 viable eggs are taken into experiment. The various concentration of EESC (10, 25, 50, 100, 150 and 200?g/ml) showed an anti-angiogenic activity based on the dose dependent manner. The result was compared with the standard (Prednisolone 5mg/ml) and control (0.9% NaCl) groups. A marked increase in the blood vessels was noted in normal saline (negative control) when compared to Prednisolone (Positive control). As the concentration of EESC increases the angiogenesis process was inhibited which revealed the anti-angiogenic activity of the EESC.