Abstract

Safaa Awad Mohammad, Tarig A.M. Hamid*, Mubarak Mustafa Elkarsany, Ahmed Elhadi Elsadig Mohammed Shazaly A. Elhassan, Sarah M. D. Ahmed and Faris Merghani Eltom

ABSTRACT

Background: methylenetetrahydrofolate reductase (MTHFR) enzyme is a catalyst in the folate metabolism pathway, The MTHFR polymorphism associated with a 50% reduction of MTHFR enzyme activity, Variations in folate metabolism including genetic polymorphisms in the key metabolic enzymes had showed influences in the MI process. Objectives: To determine association of Methylene tetrahydrofolate Reductase (MTHFR) gene C677T (rs1801113) Polymorphism among Sudanese patients with Myocardial Infarction. Material and methods: This is hospital based case control study, a total of 140 Sudanese subjects were enrolled, 70 patients with myocardial infarction and 70 age- and sex matched healthy volunteers as a control group. Genomic DNA was extracted by QIA gene kits and The SNPs genotypes were determined using polymerase chain reaction followed by restriction fragment length polymorphism method. (PCR-RFLP). Results: The frequency of the CC genotype was higher in the control group compared with patients (100%, 94%), while the CT genotype was higher in the patients (6%) and absent in control; the TT genotype was absent in both study group. C and T allele frequencies were 0.97 and 0.04, respectively in the MI group, while the frequencies of C, T Allele were 1.0 and 0.00 in the control group. No statistically significant association was reported between MTHFR polymorphic genotypes and MI (P.value= CC (0.32), CT 0.09)). Conclusion: C677T MTHFR polymorphism showed no association as genetic risk factor for MI among Sudanese patients