QUALITATIVE DETERMINATION OF ESSENTIAL OIL COMPONENTS OF STAR ANISE BY GAS CHROMATOGRAPHY(GC)
K. Suneetha*, A. Prasanna and N. Sai Vinay
ABSTRACT
Essential oils are used in various fields for different purposes. Essential oils are extracted from their specific raw materials by distillation process. The Star Anise is used as a Spice in Culinary (cooking) purposesand the essential oil of Star Anise is used in Medicinal purpose as in Aromatherapy to treat long term and short-term stress related issues. The star anise essential oil is extracted by distillation process, it is first subjected to preliminary tests such as Thin Layer Chromatography and aPhytochemical screening test for terpenoids. Further the extracted essential oil is Qualitatively Determined by using Gas Chromatography. The sample is injected through the inject port it is carried through carrier gas (mobile phase) to the column (stationary phase) and then to the detector (flame ionization detector).The result is observed in the software autochrome 300. The blank is run in which no peaks are observed ,sample is run showing many peaks it is compared with a the standard .Many peaks in the sample chromatogram indicates the presence of many components in it among them Anethol is one of the most important and abundant component of essential oil.
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