Abstract

*Rajeshwari Harode, Abhishek Banke, R B Goswami, *Durga Pandey

ABSTRACT

Objective of the work is to formulate and evaluate microemulsion containing Ciprofloxacin within oleic acid and Chitosan for improvement of antimicrobial activity of Ciprofloxacin. Optimized formulation characterized for different parameter. Optimization was done on the basis of droplets size and stability of the formulation. Formulation prepared by chitosan polysaccharide showed improved antimicrobial activity as compared to marketed formulation of the same drug concentration. In vitro drug release was also in sustained manner because 76.15 % drug release in 24 hours means chitosan also sustained release of drug from formulation whereas formulation withought chitosan showed release of 84.93 %. Droplets size is also around 257.5 ± 50?m or 894 ± 20 nm by Malvern analyser and SEM showed droplets size around 1.1 ?m. Thus it justifies term microemulsion. Drug entrapment in optimized formulation was around 63 percent, during formulation preparation different process variables also optimized such as shearing speed sonication time to reduce droplets in smaller size. We observed 4 min sonication and above 1000 rpm shearing speed is sufficient for formulation of microemulsion. Stability study showed optimized formulation was stable at 5, 25 and 40º C temperature, minor changes only observed but within acceptable limit. Thus microemulsion of CIFx containing chitosan in external phase can be use as a suitable delivery system for improvement of antimicrobial activity.