Abstract

Raghad A. H. Onsa*, Hussam Aldeen Mustafa Abdelrazig Bilal and Hamad H. A.

ABSTRACT

This study was carried out to update the occurrence of Contagious Bovine Pleuropneumonia (CBPP) in Khartoum State using isolation, molecular techniques and DNA sequencing of the causative agent. In addition to that tissues had been subjected to histopathological examination to the target sections (lungs). Mycoplasma mycoides subsp mycoides (Mmm) strain was isolated and characterized by previous methods. Using bioinformatic tools the isolated (RH) strain was confirmed to be (Mmm) and 100% similar to certain strains in gene-bank (PG1andVmm). Typical gross lesions of CBPP observed in tissue samples including expanded interlobular septa, hepatization, and fibrinonecrotic exudations were observed. Furthermore histopathological sections showed typical lesions. The sections revealed thickening of the pleura with fibrinous tissue mixed with homogenous eaosinophilic material. The alveoli are filled with oedema, fibrin with emphysema. Some dry areas showed fibrosis which is indicative of fibrinous pneumonia. These findings updated the presence of CBPP in Khartoum state using isolation and identification of the causative agent with Histopathological picture.