Abstract

Shaimaa M. Eldeighdye*, Kamal M. Ahmed, Hanaa A. El- Sayed and Tarek M. Allam

ABSTRACT

Background, hepatocellular carcinoma is the fourth cause of cancer related death, with increasing morbidity and mortality over the last decades. Plasma Activated Water (PAW) has received a great attention due to its potency in cancer treatments. Aims, the current study aims at investigating long-term effects of PAW on the viability of HepG2, as well as THLE2 to assess their potential antitumor activity in vitro. Methods, PAW was generated by exposing the tap water to three different doses of plasma. Following each exposure, PAW was divided into two aliquots; one used for treatment one day after being generated and the other aliquot was kept at room temperature for 60 days before using for treatment. HepG2 and THLE2 cells were treated using different concentration of PAW, and their viability were assessed utilizing MTT assay. Results, a significant increase in both pH and dissolved oxygen was observed after incubation for 60 days compared with incubation for one day. Meanwhile, the level of NO2 showed a slight increase. Treatment with PAW significantly reduced the viability of HepG2 cells in an exposure time dependent manner. Moreover, keeping PAW for (60 days) before treatment increased its efficiency in reducing viability of HepG2 compared to the freshly prepared PAW in the presence of cisplatin as reference. Interestingly, PAW, does not have harmful impact on normal cell lines. In conclusions, these results suggest that some plasma active species in tap water remains effective up to 60 days. PAW demonstrated a selective inhibitory effect on theHepG2 cells rather than the THLE2 cells. Furthermore, PAW, besides having significant selective lethal impact against HepG2, is economic as well impact when compared with cisplatin.