International Journal Of Modern
Pharmaceutical Research

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Science & Pharmacy Professional

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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ISSN 2319-5878
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Abstract

SIMULTANEOUS EQUATION METHOD FOR THE ESTIMATION OF AMBROXOL HYDROCHLORIDE AND DOXYCYCLINE HYDROCHLORIDE BY UV SPECTROPHOTOMETRY

Rohan T. Kshirsagar*, Ashpak M. Tamboli, Hasan I. Shaikhmulani, Harshvardhan T. Suryawanshi and Omkar V. Khandare

ABSTRACT

The simple, accurate, and precise UV spectrophotometric method has been developed for simultaneous estimation of Ambroxol Hydrochloride and Doxycycline Hydrochloride in bulk tablet formulation. Two wavelength 247 nm and 274 nm were selected for estimation of Ambroxol Hydrochloride and Doxycycline Hydrochloride by simultaneous equation method respectively. UV spectrophotometric method was developed as per ICH guideline using Methanol and Water as a Mobile phase. Ambroxol Hydrochloride and Doxycycline Hydrochloride individually follows the Beer-Lamberts Law over concentration range 2-10 & 20-100 ?g/mL, regression of coefficient was found to be r2=0.997 & r2=0.999 respectively. The percentage recovery was found to be 94.42 to 101.52 % at three different levels. The proposed method was successfully applied for determination of Ambroxol Hydrochloride and Doxycycline Hydrochloride in tablet dosage form as per ICH guideline the result of the analysis were validated statistically and were found to be satisfactory.

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