Abstract

Md Hasnat Jahan Ali, Nabaneeta Ghosh, Niharika Gupta, Moumita Tambuli, Nayanika Dey, Namrata Thakur, Ananya Bhattacharjee*

ABSTRACT

Piper betle and Ocimum sanctum leaf has long been used traditionally for its various pharmacological activities like analgesic, antidiabetic, anti-caries, cold & cough reliving as well as for its antimicrobial activity. In this study we tried to evaluate synergistic effect of combined leaf extract of both leaf. Ethanolic extract of Piper betle and Ocimum sanctum was performed using rotary evaporator. Gram Positive organism, Staphylococcus aureus andgram negative organism, E.coli was used for the evaluation of antimicrobial activity.Piper betle, Ocimum sanctum alone leaf extract and both leaves combined extract was used to check the zone of inhibition and minimum inhibitory concentration (MICs) against both gram negative and gram positive micro-organism. Alone Piper betle and Ocimum sanctum leaf extract showed significant zone of inhibition when compared to control group, whereas combined leaf extract showed moderately significant increase in zone of inhibition than the alone treated leaf extracts of Piper betle and Ocimum sanctum. Different concentration (31.25?g/ml, 62.5?g/ml, 125?g/ml, 250?g/ml, 500?g/ml, 1000?g/ml) of combined leaf extract were used for evaluation of MICs. However there was no zone of inhibition in 31.25?g/ml conc. and the minimum inhibitory concentration exhibiting zone of inhibition was at 62.5?g/ml concentration. The combined leaf extract showed extremely significant antimicrobial activity in our study.