Abstract

Rukhsar Bano*, Ramesh Kumar Gupta

ABSTRACT

This research focuses on pharmacological screening of anti-oxidant and anti-psoriatic potential of Adenium Obesum in Imquimod induced psoriasis in rats. The Hydroalcoholic flower extract of Adenium obesum (FEAO), Imquimod (API) was obtained from Glenmark Pvt Ltd Mumbai. Carbopol 934 was purchased from Sigma Aldrich Pvt Ltd. India. The fresh flowers of Adenium obesum were collected from the UP-West region and were authenticated by the botanist at CSIR New Delhi with reference no. NIScPR/RHMD/Consult/2022/4047-48. Animal House, Hygia Institute of Pharmaceutical Education & Research, Lucknow has provided rats (either sex) weighing 130-160g. The animals are kept in good health with room temp. of 25°C and 12hr light & dark cycle. Rats were divided 5 groups: Negative control- animals were applied gel base once a day, up to 15 days, Positive control- animals were applied IMQ (3.20mg) on saved dorsal skin once a day, up to 7 days, Test 1- animals were applied IMQ (3.20mg) (for 7 days) + Retino- A on saved dorsal skin once a day, up to 15 days, Test 2- animals were applied IMQ (3.20mg) (for 7 days) + FEAO gel on saved dorsal skin once a day, up to 15 days & Std.- animals were applied IMQ (3.20mg) (for 7 days) + FEAO gel on saved dorsal skin orally once a day, up to 15 days. Anti-psoriatic activity was evaluated in parameters i.e., body weight, weight of organs, determination of anti-oxidant activity, scoring of severity score PASI score (erythema, desqumation, epidermal, thickness) in Imiquimod-induced psoriatic model. In all the parameters, FEAO gel significantly demonstrated anti-psoriatic activity when compared with positive & negative control groups. In conclusion, FEAO predominantly showed anti-psoriatic potential when observed in animal studies.