Abstract

Angelin Buela Caroline M.*, Senthamizhselvi S., *Dr. B. K. Nayak and Anima Nanda

ABSTRACT

Actinomycetes are the exciting and unique group of filamentous bacteria that are well documented for their metabolic adaptability among all microbes. Bioactive feasibility of these actinobacteria facilitates their survival even in unfavourable and anguish locations. Diverse unexplored locations often demand to researchers in the hope of succeeding novel actinomycetes with constant mission that has really led to find extraordinarily productive microbial strains. In our current study, actinomycetes were isolated from different outdoor environments of Dr. Abdul Kalam Eco-garden of our KMGIPSR college campus i.e., near the pond and 20meter away from the pond and at various altitudes viz., above the ground, 5ft above the ground by employing gravity petriplate method using five different media for 5 minutes and 10 minutes each. A total of 27 isolates of the actinomycetes were recorded from both the garden environments. The maximum number of actinomycetes were recorded form 20ft away from pond in comparison to near the pond. Among the Petri plate exposer timing, 10 minute exposer was found to be good to isolate a countable number of actinomycetes in comparison to 5 minutes. Starch Casein Agar (SCA) was documented as one of the well-suited media to isolate actinomycetes from air as compared to Potato Dextrose Agar (PDA), Glycerol Asparagine Agar (GAA) and Actinomycete Isolation Agar (AIA). Isolated airborne actinomycetes were found varied configuration in their concentrations more likely at away from the pond in comparison to near the pond. Based on the antagonistic nature on agar plates, five actinomycetes were tested for their antimicrobial potential, only one actinomycetes was found active against Staphylococcus aureus, Vibrio cholera, Klebsiella pneumoniae, Pseudomonas aeruginosa and Candida albicans.