EFFECTS OF NITRATE ON HEMOGLOBIN COUNT, HISTOPATHOLOGY AND MICRONUCLEUS STUDY IN FRESH WATER FISH CATLA CATLA
*Subhashri Priyadarsini, Dr. T. Gayatri and Dr. Satya Narayan Sahoo
ABSTRACT
Water got contaminated by industrial, agricultural influence and city sewage water. Nitrate present in all industrial and mostly in agricultural waste and also city Sewage contain high level of nitrate which directly impact aquatiaquatic life. Nitrate is a major cause for the formation of met hemoglobin and shortage of hemoglobin which studied by cyanmethemoglobin method (1)20 ml of blood samples were taken in Thomas pipette at interval of 24, 48 and 72 hours. And nitrate cause stress environment which causes micronucleus and other genetic abnormalities and also a vital histological change in the gills of fish. 0, 0.5, 1.0 and 2.0 PPM nitrate dodge given to the fish and studied by conducting several tests with two way ANOVA significant variation.
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