MICROWAVE ASSISTED EXTRACTION AND SPORE GERMINATION INHIBITION ACTIVITY OF SOLANUM INDICUM
Dr. Prathima Mathias D. A.*, Santhosh D. S., Madhu M.B. and Gopi T. S.
ABSTRACT
Solanum indicum was collected from college campus. Microwave assisted Sohxlet extraction was carried out using solvents of increasing polarity i.e. petroleum ether, chloroform, ethyl acetate and ethanol. Crude extracts were subjected to phytochemical and spore germination inhibition screening in comparison to standard antifungal, water and DMF as controls. Fusarium oxysporum was inhibited to 83 % by chloroform extract, 81.92 % by Ethyl acetate extract, 80 % by Pet ether extract and only 53.91 % by ethanolic extract. Curvularia lunata was effectively inhibited by Pet ether and Chloroform extract, 83% by Ethyl acetate extract and 78% by ethanolic extract. The soil sample was examined for pH (5.45), Organic carbon (0.69%), Available Phosphorous (11 Kg acre-1), Potash (122.79 Kg acre-1), Zinc (1.3 Kg acre-1), Manganese (15.58 Kg acre-1), Copper (1.24 Kg acre-1) and Iron (9.51 Kg acre-1). The soil was found to be fertile. Chloroform extract was subjected to autobioassay by slant spray method and proved to be effective inhibitor of Curvularia lunata.
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