INVESTIGATION OF LARVICIDAL ACTIVITY OF CAESALPINIA BONDUC L. ROXB BARK
Heena Kouser S., Omkarswamy Maradimath*, Tejaswini Sm and Dr. Aman Suresh T.
ABSTRACT
The dengue virus, yellow fever, and other contagious diseases are spread by themosquito Aedes aegypti. New solutions, such as bio-larvicides, have been createdsince this mosquito has a high level of resistance to the organophosphoruslarvicides used to prevent its growth. Utilising plant extracts is quite common. Theaim of this study was to evaluate the larvicide activity of Azadirachta indica leafethanolic extract against Aedes aegypti and to characterise its main componentsusing GC-MS. The experiment used three different concentrations of ethanolicextract: 10 mg L-1, 20 mg L-1, and 50 mg L-1. A commercial larvicide consistingof Bacillus thuringiensis var. israelensis Serotype H-14 spores and endotoxiccrystals was used as a positive control, while water was used as a negative control,three times. Higher larval mortality (93%) was reported after 72 hours of incubationin the ethanolic environment 50 mg L-1 of extract was utilised; extracts at 10 mg L-1 and 20 mg L-1 resulted in 47% and 70%, respectively, larval death. The primarysubstance discovered by the GC-MS research was phytol (14. 4% area). The resultsof this study demonstrated that A. indica's ethanolic extract might kill A. aegyptilarvae. Objectives: To carry out qualitative analysis of crude extract for thepresence of phyto constituents. • To investigate the larvicidal activity of the testextract bark of Ceasalpinea bonduc. L. Roxb. Result: The medicine Ceasalpinebonduc's mortality rate. When the concentration was raised, L. Roxb barkdemonstrated an increase in mortality. When compared to low doses, high doses(mention concentration) had a higher death rate, indicating that the action was dosedependent.Standard medicine has a high death rate. Conclusion: The larvicidalaction of our test substance, an extract of Ceasalpinia bonduc. L. Roxb, is dosedependentand exhibits a high death rate at high concentrations and a low mortalityrate at low concentrations.
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