Abstract

Soumya Nijaguna Hiremath*, Jagadeesha A. S., A. Veeranagouda and Ittagi Shanmukha

ABSTRACT

Peptic ulcer is one of the major disease affecting the human population. It developsdue to the imbalance between aggressive factors like acid, pepsin, H. pylori and bilesalts and defensive factors like mucous, bicarbonate, blood flow, epithelial cellrestoration and prostaglandins. Ulcers are more common in adult males and itoccurs commonly at old age and in lower socio-economic class of individuals. Italmost affects 9.5% women population and 10.5% men. This study is designed todetermine the gastro protective activity of leaf extract of Spathodea CampanulataP. Beauv in ethanol induced ulcer model. The various extracts were prepared byusing petroleum ether, chloroform, 70% ethanol and aqueous as a solvents. The70% ethanolic extract (EELSC) was used to screen the gastro protective activitybecause of presence of higher concentration of flavonoids and polyphenols. The70% EELSC was screened for anti ulcer activity induced by ethanol. The variousrelevant parameters like ulcer index, gastric volume, gastric PH, free acidity andtotal acidity were estimated in ulcer model. In-vivo antioxidants i.e. GSH, LPO,SOD and CAT were also carried out in stomach tissue. Pretreatment with 70%EELSC exhibited significant ulcer protective property at all the tested doses of 250,333 and 500 mg/kg in ethanol induced gastric ulcer model. Anti-secretary studies inrats revealed that, the dose 333mg/kg significantly reduced total acidity, freeacidity, gastric output and increases the pH of gastric secretion as compared to theother two doses. The test extract significantly increased levels of GSH, SOD andCAT and reversed the LPO level. The gastroprotective property may be attributedto the polyphenolic compounds like flavonoids and tannins that are present in theleaves of Spathodea campanulata P. Beauv.